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Mouse Cell Line Authentication Consortium

National Institute of Standards and Technology (NIST)

The National Institute of Standards and Technology (NIST) and ATCC® have partnered in an effort to standardize mouse cell line authentication, which has resulting in the following:

  • An organized Mouse Cell Line Authentication Consortium in partnership with NIST was created.
  • Cooperative research and development agreement (CRADA) are in place between NIST and Consortium members. Each CRADA is identical, so that every laboratory is working under the exact same conditions in the agreement and is protected equally.
  • ATCC hired a Guest Researcher to work at NIST on the Mouse Cell Line Authentication Project.

The primary goals of the Consortium are:

  1. Perform validation testing to determine the informative-ness of the 19 STR makers to discriminate among mouse cell lines
  2. Collect and publish concordance data for the most commonly used mouse cell lines
  3. Deposit STR profiles for mouse cell lines into the NCBI public database
  4. Publish a written consensus standard for mouse cell line authentication by identity testing

An initial webinar was presented to the Consortium members. This webinar discussed the validation studies, as well as the CRADA agreements between NIST and the members.

The National Institute of Standards and Technology (NIST), an agency of the United States Department of Commerce, has established the Mouse Cell Line Authentication Consortium and invited organizations to participate in this Consortium. The Consortium will collaborate to obtain concordant short tandem repeat (STR) profiles for mouse cell lines, draft consensus standards for mouse cell line authentication, and create a public database of STR profiles for mouse cell lines. The Consortium was developed in collaboration with American Type Culture Collection (ATCC®). Participation in this Consortium was open to all eligible organizations and the invitation was published in the Federal Registry on October 10, 2016. Click here for more information