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Quantitative Genomic DNA from Salmonella enterica subsp. enterica serovar Typhimurium strain CDC 6516-60

14028DQ

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Quantitative Genomic DNA from Salmonella enterica subsp. enterica serovar Typhimurium strain CDC 6516-60 can be used for assay development, verification and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Product category
Bacteria
Product type
Molecular standard
Nucleic acid
Derived from
Salmonella enterica subsp. enterica serovar Typhimurium CDC 6516-60 (ATCC 14028)
Organism
Salmonella enterica subsp. enterica (ex Kauffmann and Edwards) Le Minor and Popoff serovar Typhimurium
Type strain
No
Genome sequenced strain
Yes
Applications
Assay development
Enteric disease research
Infectious disease research
Specification range
≥1 x 105 copies/μL
Volume
100 μL
Product format
Frozen
Storage conditions
-20°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
ATCC Genuine Nucleics can be used for assay development, verification and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.

Characteristics

Serotype
I 4,5,12:i:1,2 (Determined by depositor)
Comments
Genomic DNA isolated from a preparation of from Salmonella enterica subsp. enterica serovar Typhimurium strain CDC 6516-60 (ATCC 14028). The source organism is also available through the ATCC catalog.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Depositors
ATCC
Cross references
GenBank AX109618 Sequence 351 from Patent WO0123604.
GenBank AX111148 Sequence 1881 from Patent WO0123604.
GenBank AX111476 Sequence 2209 from Patent WO0123604.
GenBank U67869 Salmonella typhimurium SirA gene, complete cds.
GenBank U06133 Salmonella typhimurium ATCC 14028 isolate MS1 Tn10 insertion site.
GenBank U06126 Salmonella typhimurium ATCC 14028 isolate MS1321 Tn10 insertion site.
GenBank U06127 Salmonella typhimurium ATCC 14028 isolate MS1413 Tn10 insertion site.
GenBank U06128 Salmonella typhimurium ATCC 14028 isolate MS1523 Tn10 insertion site.
GenBank U06129 Salmonella typhimurium ATCC 14028 isolate MS1521 Tn10 insertion site.
GenBank U06130 Salmonella typhimurium ATCC 14028 isolate MS1633 Tn10 insertion site.
GenBank U06131 Salmonella typhimurium ATCC 14028 isolate MS1668 Tn10 insertion site.
GenBank U06132 Salmonella typhimurium ATCC 14028 isolate MS3493 Tn10 insertion site.
GenBank U06134 Salmonella typhimurium ATCC 14028 isolate MS2097 Tn10 insertion site.
GenBank U06135 Salmonella typhimurium ATCC 14028 isolate MS2101 Tn10 insertion site.
GenBank U06136 Salmonella typhimurium ATCC 14028 isolate MS4290 Tn10 insertion site.
GenBank U06137 Salmonella typhimurium ATCC 14028 isolate MS3747 Tn10 insertion site.
GenBank U06138 Salmonella typhimurium ATCC 14028 isolate MS5076 Tn10 insertion site.
GenBank U06139 Salmonella typhimurium ATCC 14028 isolate MS7481 Tn10 insertion site.
GenBank U06140 Salmonella typhimurium ATCC 14028 isolate MS6290 Tn10 insertion site.
GenBank U06141 Salmonella typhimurium ATCC 14028 isolate MS9020 Tn10 insertion site.
GenBank U06142 Salmonella typhimurium ATCC 14028 isolate MS8282 Tn10 insertion site.
GenBank U31849 Salmonella typhimurium pagD, envE, msgA and envF genes, complete cds.
GenBank U65941 Salmonella typhimurium O-antigen acetylase (oafA) gene, complete cds.
GenBank AF129435 Salmonella typhimurium bovine colonization factor BcfC gene, complete cds.
GenBank AF130422 Salmonella typhimurium bovine colonization factor operon, complete sequence.
GenBank AF175343 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch1c, genomic survey sequence
GenBank AF175344 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch6c, genomic survey sequence
GenBank AF175345 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch8c, genomic survey sequence
GenBank AF175357 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh2, genomic survey sequence
GenBank AF175358 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh3, genomic survey sequence
GenBank AF175359 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh4, genomic survey sequence
GenBank AF175360 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh6, genomic survey sequence
GenBank AF175361 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh8, genomic survey sequence
GenBank AF175362 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh9, genomic survey sequence
GenBank AF175346 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch14c, genomic survey sequence
GenBank AF175347 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch16c, genomic survey sequence
GenBank AF175348 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch20c, genomic survey sequence
GenBank AF175349 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch26c, genomic survey sequence
GenBank AF175350 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch36c, genomic survey sequence
GenBank AF175351 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch37c, genomic survey sequence
GenBank AF175352 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch39c, genomic survey sequence
GenBank AF175353 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch43c, genomic survey sequence
GenBank AF175354 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch44c, genomic survey sequence
GenBank AF175355 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch45c, genomic survey sequence
GenBank AF175356 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch46c, genomic survey sequence
GenBank AF175363 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh13, genomic survey sequence
GenBank AF175364 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh14, genomic survey sequence
GenBank AF175365 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh15, genomic survey sequence
GenBank AF175366 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh16, genomic survey sequence
GenBank AF175367 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh17, genomic survey sequence
GenBank AF175368 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh19, genomic survey sequence
GenBank AF175369 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh22, genomic survey sequence
GenBank AF175370 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh25, genomic survey sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Curated Citations

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Judicial Commission of the Internat. The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80. Int J Syst Evol Microbiol 55: 519-520, 2005. PubMed: 15653929

Tindall BJ, et al. Nomenclature and taxonomy of the genus Salmonella. Int J Syst Evol Microbiol 55: 521-524, 2005. PubMed: 15653930

Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

View All Curated Citations for this Product

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