Thermomicrobium roseum Jackson et al.

1. Open vial according to enclosed instructions.                            
2. Using a single tube of #655 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet. 
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth. 
5. Incubate tubes at 70°C, shaking will increase the rate of growth. 

Additional information on this culture is available on the ATCC web site at www.atcc.org.

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