Streptomyces rapamycinicus Kumar and Goodfellow

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #1877 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1877 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #334 plate.
4. Incubate at 26°C for 5-14 days.

The organism may grow more readily on agar after growth is established first in broth during the initial incubation.  The plates should be parafilmed to keep in moisture. Spore formation may take up to 14 days.

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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