Vibrio gazogenes (Harwood) Baumann et al.

1. Open vial according to enclosed instructions.
2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the broth tube for 48 to 72 hours at 26°C.
6. Transfers can now be made to other tubes of broth, slants, and/or plates.  This strain needs to establish good growth in the broth tube before additional passages are made.  Additional incubation may be required for growth on solid medium.

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