Magnetospirillum magnetotacticum (Maratea and Blakemore) Schleifer et al.

1. Thaw contents of the vial in a 37°C water bath with gentle agitation.
2. Inoculate a single 16 x 125 mm tube of #1653 broth with the contents of the thawed vial.  Be sure the entire tube is filled with medium to achieve microaerophilic conditions.
3. Incubate at 28°C to 30°C for 4 to 10 days.
4. When broth becomes cloudy and a small gray sediment is visible at the bottom of the tube, cells can be harvested.  Divide contents of the tube into two tubes and spin in a tabletop centrifuge.
5. Discard the supernatant liquid.  Do not bubble or shake cells.  Resuspend cells in a 125 mL flask filled to capacity.
6. Incubate as directed above.

For storage, centrifuge cells for 30 minutes at 7,000 rpm.  Discard the supernatant liquid.  Resuspend cells in approximately 6 ml of fresh broth.  Add an equal amount of sterile 20% glycerol.  Freeze at as low a temperature as available.

When this strain is grown under microaerophilic conditions, the cells are magnetotactic.  They have magnetite inclusions, which cause the cells to turn in a magnetic field.  This can be observed in a wet mount from a broth culture.  The strain will grow aerobically, but will not be magnetic.

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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