PHG23 Plasmid in Escherichia coli RR1
39900 ™
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Detailed product information
Characteristics
- Comments
-
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; BglI--2.4, 1.9, 0.6, 0.3; AvaI--5.1; SalI--5.1.Insert corresponds to nucleotides 39-743 of the published sequence with minor nucleotide differences in the 3' noncoding region.Insert does not contain the initiator AUG.Insert was C-tailed and cloned into the G-tailed PstI site of pBR322. The number of G/C pairs at each end of cDNA is unknown but is <50.
- Mycoplasma contamination
- Not detected
- Technical information
-
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Vector information
- Construct size (kb)
- 5.099999904632568
- Intact vector size
- 4.363
- Vector name
- pBR322
- Type of vector
- plasmid
- Construction
- pBR313
- Host range
-
Escherichia coli
- Vector end
-
PstI
- Vector information
-
Cross references: DNA Seq. Acc.: J01749
- Cloning sites
-
EcoRI; ClaI; HindIII; EcoRV; BamHI; SphI; SalI; XmaIII; NruI; BspMI; BsmI; StyI; AvaI; BalI; BspMII; PvuII; Tth111I; NdeI; AflIII; PpaI; PstI; PvuI; ScaI; SspI; AatII
- Markers
-
ampR; tetR
- Replicon
- pMB1
Insert information
- Insert size (kb)
- 0.69999999999999996
- Type of DNA
- cDNA
- Insert source
- T-cells, peripheral blood lymphocytes
- Insert tissue
-
T-cells, peripheral blood lymphocytes
- Insert information
- Genomic copy number: unique
- Genome
- human
- Chromosome
-
55 q23-q31
- Gene name
- colony stimulating factor 2 (granulocyte-macrophage)
- Gene product
- colony stimulating factor 2 (granulocyte-macrophage) [CSF2]
- Gene symbol
- CSF2
- Contains complete coding sequence
- Unknown
- Insert end
-
Modification: GC tails
Handling information
- Host
-
Escherichia coli RR1
- Medium
- Temperature
-
37°C
- Handling notes
-
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1;
BglI--2.4, 1.9, 0.6, 0.3; AvaI--5.1; SalI--5.1.
- ATCC staff
Insert corresponds to nucleotides 39-743 of the published sequence with minor
nucleotide differences in the 3' noncoding region. Insert does not contain the
initiator AUG. Insert was C-tailed and cloned into the G-tailed PstI site of
pBR322. The number of G/C pairs at each end of cDNA is unknown but is <50.
- personal communication
.tail_inf
.patent
History
- Depositors
- Immunex Corporation, S Gillis, Immunex Corporation
- Patent depository
-
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
- Patent number
-
5,032,676
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
- Disclosures
-
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Curtis BM, et al. Fusion proteins comprising GM-CSF and IL-3. US Patent 5,073,627 dated Dec 17 1991
Curtis BM, et al. DNA sequences encoding fusion proteins comprising GM-CSF and IL-3. US Patent 5,108,910 dated Apr 28 1992
Steven Gillis, personal communication
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