Gluconacetobacter diazotrophicus (Gillis et al.) Yamada et al.

Potential plant growth promoting strain; Biofertilizer

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #1 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #1 plate and/or #1 agar slant.
4. Incubate at 30°C for 24-72 hours.

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