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pHcGAP

57091

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
standard for analysis of gene expression glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase

Characteristics

Comments
The PstI site at the 3' end of the insert was destroyed during cloning. The retained PstI site is 750 bp from the EcoRI site. The insert contains a HindIII and a XbaI site.
Restriction digests of the clone give the following sizes (kb): PstI--5.55; HindIII--4.5, 1.06; RsaI--2.1, 1.4, 0.86, 0.65, 0.56; XbaI/PstI--4.7, 0.8; BglI--2.9, 2.2, 0.4, 0.2; BglI/PstI--2.2, 1.8, 1.0, 0.4, 0.2.
Includes 31 bp of 5' and 216 bp of 3' untranslated sequence, and a complete 1008 bp coding sequence. The polyadenylation signal AATAA is 15 bp upstream from poly(A) site.
The XbaI site is about 780 bp from the 5' PstI site. A PstI/XbaI double digest should give a probe containing the 5' non-coding sequences and sequences encoding about the first 250 amino acids.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Thirty hybridizing bands are detected in genomic blots at 37C, 50% formamide, 5X SSC; 100 bands are detected when screening a genomic library under the same conditions.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.599999904632568

Insert information

Insert size (kb)
1.2
Type of DNA
cDNA
Insert source
fetal liver
Insert tissue
fetal liver
Target gene
glyceraldehyde-3-phosphate dehydrogenase
Gene product
glyceraldehyde-3-phosphate dehydrogenase(glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase) [GAPD]

History

Cross references
GenBank M17851

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Tso JY, et al. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene. Nucleic Acids Res. 13: 2485-2502, 1985. PubMed: 2987855

Norgard MV, et al. Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappaB. Infect. Immun. 64: 3845-3852, 1996. PubMed: 8751937

Filler SG, et al. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect. Immun. 64: 2609-2617, 1996. PubMed: 8698486

Barbara Weiffenbach, personal communication

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