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pV11SPRS [TRGV11]

59604

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.27; EcoRI--3.0; HindIII--3.0; RsaI--2.0, 0.66, 3.2; RsaI/HindIII--1.7, 0.66, 0.32(doublet).
Codominant segregation of the SacI polymorphism demonstrated in one family; demonstrated in 3 families for the TaqI polymorphism.
The A2 allele of the SacI polymorphism results from the presence of an additional SacI site at 5.8 kb in 3' of V11.
Run on 0.8% agarose gels and hybridize under low stringency conditions.
Subcloned from lambdaJM15. Contains the 5' region of the gene.
The probe detects the following invariant genomic fragments (kb): HindIII--19; EcoRI--11.2; PvuII--4.0.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.0

Insert information

Insert size (kb)
0.27000000000000002
Type of DNA
genomic
Gene product
T cell receptor, gamma, variable region V11 [TCRGV11]
Alleles
F2, F1, E1, E2

Handling information

History

Depositors
M Lefranc
Cross references
GenBank X07207
GenBank 169707

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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Frequently Asked Questions

References

Curated Citations

Huck S, et al. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 7: 719-726, 1988. PubMed: 2969332

Ghanem N, et al. SacI poplymorphism defined by human T-cell receptor gamma (TCRG) variable gene VII. Nucleic Acids Res. 17: 1271, 1989. PubMed: 2564181

Ghanem N, et al. A TaqI RFLP of the human T-cell receptor gamma (TCRG) variable gene V11. Nucleic Acids Res. 17: 1270, 1989. PubMed: 2564180

Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

View All Curated Citations for this Product

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