Salmonella enterica subsp. salamae (Le Minor et al.) Le Minor and Popoff

1. Open the freeze-dried vial according to enclosed instructions.
2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #18 slant, and/or plate
5. Incubate all tubes and plates at 37°C for 24 hours.

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