YAC construction vectors kit
77191 ™
This is a kit of the left and right arms of a YL-type Saccharomyces cerevisiae yeast artificial chromosome vector (YAC). These YL-type shuttle vectors are useful for construction of genomic libraries. Each plasmid is digested with ClaI and the cloning enzyme. To recover both ends of a YAC insert as plasmid, DNA of a YAC-containing strain is digested to completion with BamHI, SphI or SacI, diluted, ligated and transformed into E. coli (to ampicillin resistance). The insert of a resultant plasmid (which includes the T7 promoter) extends from an end of the YAC insert to the first site of the enzyme used for digestion.