pGEX-KN
77332 ™
Cloning into this vector requires amplification of the gene using oligonucleotides prepared as in the reference and encoding the first 4 amino acids of a thrombin recognition sequence.
Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine ?kinker? and a NotI site. The order of the major features in this plasmid is: pMB1 ori ? lacIV ? lacZ ? tac promoter ? GST ? glycine ?kinker? ? NotI/MCS/EcoRI ? ampR.