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pHG4.5

79002

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): NcoI/SalI--4.5, 3.0; EcoRI--7.5; PstI--4.25, 2.25, 0.9; SacI--4.4, 1.95, 0.65, 0.4; SmaI--7.5.
The insert contains the following restriction sites (kb from the 5' end): BglI--3.03; BglII--3.1; EcoRI--4.33; PstI--2.2, 2.4, 3.23, 3.28; PvuII--3.21, 3.28, 4.17; SacI--2.43, 2.58, 3.0; SmaI--2.33.
An 0.8 kb PstI fragment of the insert hybridizes to the following fragments of human genomic DNA (kb): EcoRI--4.7; HindIII--7.2; BamHI--10.4; BglII--4.0; StyI--1.3. Detects a 9.0 kb EcoRI fragment of hamster genomic DNA.
Contains a complete exon 2 (2.5 kb) plus upstream and downstream sequences. Exon 2 contains most of the 5' untranslated region, the complete coding sequence (nt 2182-3519 of the sequence record) and the complete 3' untranslated region.
Derived from pHG15 which was constructed by inserting a SalI fragment from an lambdaEMBL3 library into pGEM-5Z. pHG4.5 is a circularized NcoI fragment from pHG15, and thus retains lambdaEMBL3 sequences from SalI to the 5' end of the insert.
The SP6 promoter is adjacent to the 5' end of the insert.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
7.199999809265137
Intact vector size
3.000
Vector name
pGEM-5Zf(+)
Type of vector
phagemid
Vector end
NcoI; SalI
Markers
ampR

Insert information

Insert size (kb)
4.5
Type of DNA
genomic
Insert source
chronic myeloid leukemia
Insert tissue
chronic myeloid leukemia
Insert information
DESCRIPTION OF INSERT COMPONENT:
beta-1,2-N-acetylglucosaminyltransferase
Insert 5' end:
Insert 3' end:
Cross references: DNA Seq. Acc.: M61829
Genome
Homo sapiens
Chromosome
5
5
Gene name
mannosyl (alpha-1,3-)-glycoprotein
Gene product
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [MGAT]
Gene symbol
MGAT1; GLYT1; MGAT
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): NcoI/SalI--4.5,
3.0; EcoRI--7.5; PstI--4.25, 2.25, 0.9; SacI--4.4, 1.95, 0.65, 0.4; SmaI--7.5.
- ATCC staff

The insert contains the following restriction sites (kb from the 5' end):
BglI--3.03; BglII--3.1; EcoRI--4.33; PstI--2.2, 2.4, 3.23, 3.28; PvuII--3.21,
3.28, 4.17; SacI--2.43, 2.58, 3.0; SmaI--2.33.
- GenBank/EMBL/DDBJ

The SP6 promoter is adjacent to the 5' end of the insert.
- personal communication

Contains a complete exon 2 (2.5 kb) plus upstream and downstream sequences. Exon
2 contains most of the 5' untranslated region, the complete coding sequence (nt
2182-3519 of the sequence record) and the complete 3' untranslated region.
- Biochem. Biophys. Res. Commun. 176: 608-615, 1991

An 0.8 kb PstI fragment of the insert hybridizes to the following fragments of
human genomic DNA (kb): EcoRI--4.7; HindIII--7.2; BamHI--10.4; BglII--4.0;
StyI--1.3. Detects a 9.0 kb EcoRI fragment of hamster genomic DNA.
- Biochem. Biophys. Res. Commun. 176: 608-615, 1991

Derived from pHG15 which was constructed by inserting a SalI fragment from an
lambdaEMBL3 library into pGEM-5Z. pHG4.5 is a circularized NcoI fragment from
pHG15, and thus retains lambdaEMBL3 sequences from SalI to the 5' end of the
insert.
- Biochem. Biophys. Res. Commun. 176: 608-615, 1991


More information may be available from ATCC (http://www.atcc.org or 703-365-2620).

History

Depositors
H Schachter
Cross references
GenBank M61829

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

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Frequently Asked Questions

References

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