182
79015 ™
- Organism
-
Homo sapiens, human
- Clone type
- Clone
- Applications
-
Molecular biology
- Product format
- Freeze-dried
- Shipping information
- Rehydrate with TE
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Detailed product information
Characteristics
- Comments
-
Restriction digests of the clone give the following sizes (kb): PstI--4.2, 4.2, 3.0, 3.0, 3.0, 2.35, 2.05, 1.95, 1.9, 1.7, 1.5, 1.35, 1.25, 1.1, 0.92, 0.9, 0.85, 0.85, 0.8, 0.76, 0.72, 0.48, 0.42, 0.35;PvuII--4.6, 4.4, 4.1, 3.7, 3.7, 3.2, 2.2, 2.0, 1.9, 1.65, 1.25, 1.25, 1.0, 1.0, 0.75, 0.65, 0.6, 0.46, 0.4, 0.4; EcoRI--12.0, 8.0, 7.0, 4.5, 3.3, 1.65, 0.84; EcoRI/BamHI--8.4, 8.4, 4.5, 4.3, 3.65, 2.25, 1.85, 1.78, 1.7, 1.15, 0.8;EcoRI/PstI--4.2, 3.3, 3.0, 3.0, 2.35, 2.2, 1.75, 1.55, 1.35, 1.2, 1.15, 1.1, 1.0, 0.95, 0.95, 0.84, 0.75, 0.68 +smaller.The 4428 nt sequenced region includes the following restriction sites (nt from the 5' end): PstI--531, 642, 1011 (all intron 1), 1850 (intron 2); 3218 (intron 3); EcoRI--4211 (exon 4).IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.The sequence record begins at a Sp1 binding site consensus approximately 260 bp 5' to the initiator ATG.Contains the complete gene (4 exons).Introns 2 and 3 contain Alu repeats.
- Mycoplasma contamination
- Not detected
Vector information
- Construct size (kb)
- 40.0
- Intact vector size
- 5.400
- Vector name
- pJB8
- Type of vector
- cosmid
- Construction
- HomerI
- Host range
-
Escherichia coli
- Vector end
-
BamHI
- Cloning sites
-
BamHI; EcoRI
- Markers
-
ampR
- Replicon
- pMB1
Insert information
- Insert size (kb)
- 35
- Type of DNA
- genomic
- Insert source
- leukocyte
- Insert tissue
-
leukocyte
- Insert information
-
DESCRIPTION OF INSERT COMPONENT:Genomic copy number: uniqueCross references: DNA Seq. Acc.: J05303
- Genome
- Homo sapiens
- Chromosome
-
2222 q12-q13.1
- Gene name
- lectin, galactoside-binding, soluble, 1
- Gene product
- lectin, galactoside-binding, soluble, 1 [LGALS1]
- Gene symbol
- LGALS1
- Contains complete coding sequence
- Unknown
- Insert end
-
MboI
Handling information
- Handling notes
-
Restriction digests of the clone give the following sizes (kb): PstI--4.2, 4.2,
3.0, 3.0, 3.0, 2.35, 2.05, 1.95, 1.9, 1.7, 1.5, 1.35, 1.25, 1.1, 0.92, 0.9,
0.85, 0.85, 0.8, 0.76, 0.72, 0.48, 0.42, 0.35; PvuII--4.6, 4.4, 4.1, 3.7, 3.7,
3.2, 2.2, 2.0, 1.9, 1.65, 1.25, 1.25, 1.0, 1.0, 0.75, 0.65, 0.6, 0.46, 0.4, 0.4;
EcoRI--12.0, 8.0, 7.0, 4.5, 3.3, 1.65, 0.84; EcoRI/BamHI--8.4, 8.4, 4.5, 4.3,
3.65, 2.25, 1.85, 1.78, 1.7, 1.15, 0.8; EcoRI/PstI--4.2, 3.3, 3.0, 3.0, 2.35,
2.2, 1.75, 1.55, 1.35, 1.2, 1.15, 1.1, 1.0, 0.95, 0.95, 0.84, 0.75, 0.68
+smaller. IMPORTANT: To prevent amplification of a rearranged and/or deleted
cosmid, we recommend streaking on LB + amp plates at 30C and picking small
colonies for liquid culture.
- ATCC staff
The sequence record begins at a Sp1 binding site consensus approximately 260 bp
5' to the initiator ATG.
- Biochemistry 30: 82-89, 1991
Contains the complete gene (4 exons).
- Biochemistry 30: 82-89, 1991
Introns 2 and 3 contain Alu repeats.
- Biochemistry 30: 82-89, 1991
The 4428 nt sequenced region includes the following restriction sites (nt from
the 5' end): PstI--531, 642, 1011 (all intron 1), 1850 (intron 2); 3218 (intron
3); EcoRI--4211 (exon 4).
- Biochemistry 30: 82-89, 1991
History
- Cross references
-
GenBank J05303
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566
Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031
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