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cRH15A

79939

Detects:
Genome: Homo sapiens
Gene symbol: LDLR
Type of nucleic acid: genomic
Restriction enzyme: KpnI+SacI
Invariant bands (kb): several
Number of alleles: 2
Type of polymorphism: site
Alleles:
Allele Freq. Size (kb) Strains
S2 0.50 2.0
S1 0.50 2.05
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
The whole cosmid has been used as the probe.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 8.4, 3.8, 3.1, 2.0, 1.1, 0.98; BglII--15.0 (doublet), 6.0, 5.2, 4.4, 1.6, 1.3; EcoRI--15.0, 12.0, 9.4, 8.8, 2.3, 1.4; HindIII--19.0, 15.0, 8.2, 3.4, 2.5;
BstEII--11.0, 9.4, 8.4 (doublet), 5.4, 4.5, 2.3.
The whole cosmid has been used as the probe.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Enzymes not detecting polymorphism: BglI, BstEII, BxtXI, DraI, EcoRV, HincII, XmnI, MspI, TaqI, BamHI, BglII, PstI, EcoRI, HindIII.
Polymorphisms were also not detected with SacI or KpnI in those individuals demonstrating polymorphism with SacI/KpnI-digested DNA.
Mycoplasma contamination
Not detected

Vector information

Vector name
unknown
Type of vector
cosmid
Host range
Escherichia coli
Markers
ampR

Insert information

Insert size (kb)
40
Type of DNA
genomic
Insert information
DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
hypercholesterolemia)
Cross references:
Genome
Homo sapiens
Chromosome
19
19 p13.2
Gene name
low density lipoprotein receptor (familial
Gene product
low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR]
Gene symbol
LDLR
Alleles
A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1
Contains complete coding sequence
Unknown

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI--20.0,
9.0, 8.4, 3.8, 3.1, 2.0, 1.1, 0.98; BglII--15.0 (doublet), 6.0, 5.2, 4.4, 1.6,
1.3; EcoRI--15.0, 12.0, 9.4, 8.8, 2.3, 1.4; HindIII--19.0, 15.0, 8.2, 3.4, 2.5;
BstEII--11.0, 9.4, 8.4 (doublet), 5.4, 4.5, 2.3. IMPORTANT: To prevent
amplification of a rearranged and/or deleted cosmid, we recommend streaking on
LB + amp plates at 30C and picking small colonies for liquid culture.
- ATCC staff

The whole cosmid has been used as the probe.
- Nucleic Acids Res. 17: 470, 1989

Enzymes not detecting polymorphism: BglI, BstEII, BxtXI, DraI, EcoRV, HincII,
XmnI, MspI, TaqI, BamHI, BglII, PstI, EcoRI, HindIII.
- Nucleic Acids Res. 17: 470, 1989

Polymorphisms were also not detected with SacI or KpnI in those individuals
demonstrating polymorphism with SacI/KpnI-digested DNA.
- Nucleic Acids Res. 17: 470, 1989

Legal disclaimers

Intended use
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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Hegele RA, et al. Three RFLPs upstream of the low density lipoprotein receptor (LDLR) gene. Nucleic Acids Res. 17: 470, 1989. PubMed: 2563158

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