lambdafoo
87099 ™
- Clone type
- Vector
- Applications
-
Molecular biology
- Product format
- Freeze-dried
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Detailed product information
General
- Specific applications
-
expression vectorvector for constructing cDNA librariesvector permitting construction of fusion proteinsvector permitting visual detection of recombinants
Characteristics
- Comments
-
Restriction digests of the clone give the following sizes (kb): BamHI--33.0, 9.4; EcoRI--33.0, 9.4; BglII--22.0, 8.8, 4.8, 4.6, 3.1; PstI--9.6, 9.0, 4.6, 3.2, 2.9, 2.8, 2.4, 2.2, 1.9, 1.9, 1.5.Vector allowing expression of cloned inserts as a fusion protein on the phage particle surface. Inserts are fused to the C-terminus of a truncated phage tail protein by a peptide linker.Presence of the lacZalpha coding sequence and ribosome binding site allow blue-white color detection of recombinants, as well as allowing expression of a cloned insert separate from the phage tail protein.The Pro-Thr box encodes alternating prolyl and threonyl residues, which form a link between the N-terminal phage tail protein and the foreign protein.The linker resembles the hinge-region of IgA1 and allows separation of the foreign protein from the phage particles by digestion with enzymes such as Cellulomonas fimi protease or collagenase.Production of large amounts of fusion protein may inhibit phage assembly. A host allowing low efficiency suppression of the amber mutation is recommended.
- Mycoplasma contamination
- Not detected
Vector information
- Construct size (kb)
- 43.0
- Intact vector size
- 43.000
- Vector name
- lambdafoo (phage, lambda - replacement)
- Type of vector
- phage
- Construction
- lambda2001, lambda gene V
- Host range
-
Escherichia coli
- Vector information
-
other: amber stop codon, ->other: Pro-Thr box encoding a linker peptide, ->other: amber stop codonother: Pro-Thr box encoding a linker peptide
- Cloning sites
-
HindIII; BamHI; SacI; EcoRI
- Coding sequence
- gene V N-terminal 176 aa, ->; gene V N-terminal 176 aa
- initiation codon
- ATG
- Insert detection
- lacZ', ->
- MCS
- HindIII...EcoRI, ->
- Polylinker sites
-
HindIII; BamHI; SacI; EcoRI
- Restriction sites
-
SfiI; SfiI
- Ribosome binding site
- Shine-Dalgarno sequence
Handling information
- Medium
- Temperature
-
37°C
- Handling notes
-
Restriction digests of the clone give the following sizes (kb): BamHI--33.0,
9.4; EcoRI--33.0, 9.4; BglII--22.0, 8.8, 4.8, 4.6, 3.1; PstI--9.6, 9.0, 4.6,
3.2, 2.9, 2.8, 2.4, 2.2, 1.9, 1.9, 1.5.
- ATCC staff
Vector allowing expression of cloned inserts as a fusion protein on the phage
particle surface. Inserts are fused to the C-terminus of a truncated phage tail
protein by a peptide linker.
- Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994
Presence of the lacZalpha coding sequence and ribosome binding site allow
blue-white color detection of recombinants, as well as allowing expression of a
cloned insert separate from the phage tail protein.
- Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994
The Pro-Thr box encodes alternating prolyl and threonyl residues, which form a
link between the N-terminal phage tail protein and the foreign protein.
- Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994
The linker resembles the hinge-region of IgA1 and allows separation of the
foreign protein from the phage particles by digestion with enzymes such as
Cellulomonas fimi protease or collagenase.
- Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994
Production of large amounts of fusion protein may inhibit phage assembly. A
host allowing low efficiency suppression of the amber mutation is recommended.
- Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994
History
- Depositors
- IN Maruyama
- Special collection
-
NCRR Contract
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Maruyama IN, et al. Lambda foo: a lambda phage vector for the expression of foreign proteins. Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994. PubMed: 8058794
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