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pGIBS-PHE

87483

Expression control clone for microarrays.
Organism
Bacillus subtilis subsp. subtilis (Ehrenberg) Cohn
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
Shipping information
Escherichia coli containing the phagemid in glycerol stock
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).

Characteristics

Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 2017 - 3334 of Genbank M24537.
The insert contains the following restriction sites (approximate kb from the 5' end): XmnI--0.36; AvaI--0.89; EcoRI--1.05.
Restriction digests of the clone give the following sizes (kb): NotI--4.4; NotI/XhoI--3.0, 1.4; XhoI--4.4.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.2
Intact vector size
2.961
Vector name
pBluescript II KS+
Type of vector
phagemid
Vector end
XhoI; NotI
Vector information
Excise insert: XhoI+NotI
Promoter giving the sense strand:T3
Promoter giving the antisense strand: T7
Markers
ampR
Promoters
T3; T7

Insert information

Insert size (kb)
1.3200000000000001
Insert information
Gene: pheB
Source: Bacillus subtilis
Genbank accession: M24537 (Nucleotides 1-1318 of the insert correspond to nucleotides 2017-3334 of M24537)
Insert end (5’): XhoI linker
Insert end (3’): BamHI/poly(A)/NotI linker

Handling information

Host
Escherichia coli DH5
Medium
Temperature
37°C
Handling procedure
Transfer a loopful to a test tube containing 5 mL LB+50 µg/mL of ampicillin broth. A loopful of culture can also be streaked on an LB + amp agar plate. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures. 
Handling notes
Restriction digests of the clone gave the following sizes   (in kb):  NotI/XhoI – 3.0, 1.4 ; NotI – 4.4 ; XhoI –  4.4.
-–ATCC Staff  

History

Depositors
M Follettie
Special collection
NCRR Contract
Cross references
GenBank M24537

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

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Frequently Asked Questions

References

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