Haemophilus influenzae (Lehmann and Neumann) Winslow et al.

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #2167 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2167 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
3. Use several drops of the primary broth tube to inoculate a #814 plate and/or #814 agar slant.
4. Incubate at 37°C for 24-48 hours in an atmosphere of 5% CO₂.

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