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clone 2.72F, #19

95490

Detects:
Genome: Homo sapiens
Tissue: fibroblast
Gene symbol: ITGA2
Type of nucleic acid: mRNA
Invariant bands (kb): 8.0
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 3.0, 1.8; HindIII--3.2, 3.2, 2.1; XbaI--8.4; XhoI--4.8, 3.5; KpnI--4.8, 3.5.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.79, BamHI--1.86; HindIII--2.02, 5.2.
The coding region is nt 49-3594.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
8.329999923706055
Intact vector size
2.964
Vector name
pBluescript KS+
Type of vector
phagemid
Construction
pUC19
Host range
Escherichia coli
Vector end
EcoRI closer to XhoI site; EcoRI
Cloning sites
SacII; XmaII; NotI; XbaI; SpeI; BamHI; SmaI; PstI; EcoRI; EcoRV; HindIII; ClaI; SalI HincII AccI; XhoI; DraII; ApaI; KpnI
Enhancer
none
Insert detection
lacZ'
Markers
ampR
Polylinker sites
SEE COMMENTS
Promoters
T7; T3; lac
Replicon
pMB1; f1
Terminator
none

Insert information

Insert size (kb)
5.367
Type of DNA
cDNA
Insert source
lung fibroblast IMR-90 cell line
Insert tissue
lung fibroblast IMR-90 cell line
Insert information
DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
receptor)
Cross references: DNA Seq. Acc.: M28249
Nucleotides 1-5367 of the insert correspond to
nucleotides 2-5368 of M28249.
Genome
Homo sapiens
Chromosome
5
5 q23-q31
Gene name
integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2
Gene product
integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)(collagen receptor, platelet gpIa/IIa) [ITGA2]
Gene symbol
ITGA2
Contains complete coding sequence
Yes
Insert end
Modification: EcoRI linker

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 3.0,
1.8; HindIII--3.2, 3.2, 2.1; XbaI--8.4; XhoI--4.8, 3.5; KpnI--4.8, 3.5.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): EcoRI--1.79, BamHI--1.86; HindIII--2.02, 5.2.
- J. Cell Biol. 109: 397-407, 1989

The coding region is nt 49-3594.
- J. Cell Biol. 109: 397-407, 1989

History

Depositors
Q Yuan, ME Hemler
Cross references
GenBank M28249

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

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Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Takada Y, Hemler ME. The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain. J. Cell Biol. 109: 397-407, 1989. PubMed: 2545729

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