Helicobacter mastomyrinus

1. This organism is shipped frozen on dry ice.  Just prior to use, thaw vial in water at approximately 37°C.  When thawed, a drop of the suspension may be used to do an immediate wet mount to observe the unique morphology of this organism and verify its viability by checking for motility.
2. Aseptically transfer the thawed suspension into a #260 broth tube (5-­6 mL).  This broth can now be used to inoculate an agar slant(s), plate(s), additional broth tube(s), or the preferred biphasic culture.
3. To obtain a biphasic culture, add 0.6 mL of the suspension to a medium #260 slant.  The resulting pool at the bottom of the slant is where the best, most rapid growth will occur.
4. Incubate at 37°C under microaerophilic conditions using an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method, to obtain microaerophilic conditions.  Incubate slant with cap loose.
5. Within 5 days of incubation, good growth should be obtained in the broth pool at the bottom of the slant.    Further subcultures can be made using broth pool as the inoculum source.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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