Thermocrinis minervae Caldwell et al.

BAA-1533 ^™

Product category: Bacteria
Product type: Extremophile
Strain designation: CR11
Type strain: Yes
Isolation source: Terrestrial hot spring in Rincon Volcano
Geographical isolation: Costa Rica
Product format: Frozen
Storage conditions: -80°C or colder

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Preceptrol: No

Characteristics

Extremophile type: Sulfur oxidizer

Thermophile

Handling information

Medium: ATCC Medium 2804: MJTSO Medium
Temperature: 70°C
Atmosphere: 80% H₂, 20% CO₂ (5 psi)
Handling procedure: 1. Sterilize the top of the Balch tube by spraying it with 70% ethanol and them flaming the top
2. If needed, exchange the gas in the test tube for 80% N₂ 20% CO₂; do not over pressurize. Using a 1 ml syringe tipped with 22-gauge needle, add 0.1 ml 1 M thiosulfate to each tube of broth to be inoculated. If the balch tube contains 8 ? 10 ml of broth, add 3 ml air with a 1 ml syringe tipped with 22-gauge needle.
3. When the balch tube is ready to inoculate, thaw the frozen vial at room temperature.
4. For inoculation, using a 1.0 ml syringe tipped with 22-gauge needle, withdraw the cell suspension from the vial and transfer it to the broth. Additional tubes of #2804 can be inoculated by transferring 0.5 ml of the primary broth using good technique. Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37°C. Incubate the anaerobic tube at 70°C to 75°C.
5. Growth should be detected in the #2804 broth within 48 to 72 hours. There should be no growth detected on the aerobic plate.
Handling notes: Cells are rods that occur singly and are motile. Good growth should be detected within 48 to 72 hours.
This organism is reported to grow chemolithoautrophically with H₂ or thisulfate as the electron donors, and O₂(up to 16%) as the electron acceptor. It is also reported to utilize yeast extract, mannose, glucose, maltose, succinate, peptone, Casamino acids, starch, citrate, and CO₂ as carbon sources.
Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

History

Depositors: AL Reysenbach
Type of isolate: Environmental
Cross references: GenBank AM260555 Aquificaceae bacterium CR11 partial 16S rRNA gene, strain CR11

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Caldwell SL, et al. Thermocrinis minervae sp. Nov., a hydrogen- and sulfur-oxidizing thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring. Int. J. Syst. Evol. Microbiol. 60(2): 338-343, 2010. PubMed: 19651724

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