Blautia wexlerae Liu et al.

1. Open thawed vial.
2. Aseptically transfer the entire contents to a 5-6 mL tube of #2722 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
4. Incubate in an anaerobic atmosphere at 37°C for 24-72 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

• Loose screw caps on test tubes in an anaerobic chamber
• Loose screw caps on test tubes in an activated anaerobic gas pack jar
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

Growth in broth is typically observed in 24 hrs and may overgrow if allowed to incubate longer. Culture on agar may take 48-72 hours to achieve robust colony growth.

Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. 

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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