Photorhabdus heterorhabditis Ferreira et al.

1. Open vial. 
2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 - 48 hours.

Colonies on #18 agar are yellow, opaque, umbonate, and irregular.

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