Thermococcus siculi Grote et al.

BAA-270 ^™

Product category: Archaea
Product type: Extremophile
Strain designation: DSM 12349 [RG-20]
Type strain: Yes
Isolation source: Deep-sea hydrothermal vent, Okinawa Trough
Product format: Freeze-dried

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: Biotechnology
Preceptrol: No

Characteristics

Extremophile type: Sulfate reducer

Thermophile

Handling information

Medium: ATCC Medium 2104: Marine Thermococcus medium
Temperature: 80°C
Atmosphere: 80% N₂, 20% CO₂
Handling procedure: 1.       Open vial according to enclosed instructions.
2. Using a single tube of #2104 broth (5 to 6 ml) under anaerobic conditions, withdraw approximately 0.5 to
1.0 ml with a Pasteur or 1.0 ml pipette. Re-hydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well. Take 0.25 ml of this tube and transfer it to a second tube. Mix well.
4.       After 24 Hours of incubation, turbidity should be observed.
5.       Transfers can now be made to other tubes of broth, slants, and/or plates.
ANAEROBIC CONDITIONS:
          Anaerobic conditions for transfer may be obtained by either of the following:
·   Use of an anaerobic gas chamber, or
·   Placement of test tubes under a gassing cannula system hooked to anaerobic gas or.
·   Exchanging the headspace in stoppered culture tubes for the appropriate anaerobic gas
          Anaerobic conditions for incubation may be obtained by any of the following:
·   Loose screw‑caps on test tubes in anaerobic chamber,
·   Loose screw‑caps on test tubes in an activated anaerobic Gas Pak jar, or
·   Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes: The cells are irregular nonmotile cocci. Anaerobic techniques utilizing Hungate tubes is recommended because
growth is enhanced when tubes are pressurized (20-40 psi).

History

Deposited as: Thermococcus siculi Grote et al.
Depositors: DSMZ
Chain of custody: ATCC <-- DSMZ <-- G. Antranikian
Type of isolate: Environmental
Cross references: GenBank AB010893 16S rDNA sequence

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Grote R, et al. Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. Extremophiles 3: 55-62, 1999. PubMed: 10086845

type strain

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