Sulfurihydrogenibium subterraneum Takai et al.
BAA-562 ™
- Product category
-
Bacteria
- Product type
-
Extremophile
- Strain designation
- HGMK-1 [DSM 15120, JCM 11477]
- Type strain
- Yes
- Isolation source
- Hot aquifer water samples from Hishikari gold mine; isolated March 2001 by Ken Takai
- Geographical isolation
- Japan; Kagoshima
- Product format
- Freeze-dried
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
Biotechnology
- Preceptrol
- No
Characteristics
- Extremophile type
-
Sulfur oxidizerThermophile
Handling information
- Medium
- Temperature
-
60°C
- Atmosphere
-
Anaerobic
- Handling procedure
-
1. Sterilize the top of the Balch tube by spraying it with 70% ethanol and then flame the top.
2. If needed exchange the gas in the test tube for 100% N2, 80% N2-20%CO2. When thiosulfate is the electron donor the culture can be grown under aerobic conditions (N2-CO2-O2, 65:15:20).
3. When the Balch tube is ready to inoculate, open the vial according to enclosed instructions.
4. For inoculation, use a 1.0 ml syringe tipped with 22 gauge needle. Withdraw 0.5 ml of Medium #2346 and use this to rehydrate the freeze-dried pellet. Transfer the rehydrated cell suspension back to a tube of #2346 broth and incubate at 60oC. Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37oC. Inoculate a nonselective anaerobic and aerobic broth and incubate at 60oC
5. Growth should be detected in the #2346 broth within 24 to 48 hours. There should be no growth detected on the aerobic plate. There should be no growth in the nonselective aerobic or anaerobic broth.
- Handling notes
-
With hydrogen or thiosulfate as the electron donor this strain was able to utilize molecular oxygen, nitrate, soluble ferric citrate, insoluble ferrihydrite iron (III), arsenate, selenate and selenite.
Growth will be detected within 24 to 48 hours by turbidity that settles at the bottom of the test tube. The turbidity of the culture increases form 24 to 48 hours but the cell density does not.
The cells are Gram negative motile rods with a polar flagella. The cells are typically in short chains.
Once growth has been established, the culture should be transferred every 24 to 48 hours when maintained at 60oC. The culture can be maintained at 4oC for up to 1 week..
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Resolution: 1000X
History
- Deposited as
- Sulfohydrogenobium subterraneus
- Depositors
- K Takai
- Chain of custody
- K Takai
- Year of origin
- 2001
- Special collection
-
NSF - Bacteriology
- Cross references
-
GenBank AB071324 Aquificales str. HGM-K1 gene for 16S rRNA, partial sequence.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
Need assistance with this product? Contact our Technical Support team.
Telephone
US and Puerto Rico
800-638-6597
Outside the US
+1-703-365-2700
Hours of Operation
Monday-Friday
9:00am - 5:00pm
US Eastern Time
US Eastern Time
