Prostate Epithelial Cell Basal Medium

Prostate Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC^® Primary Cell Solutions™ system. This serum-free system is designed to support prostate epithelial cells derived from normal human prostate. Prostate Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of prostate epithelial cells, Prostate Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of prostate epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

1. For prostate epithelial cells derived from prostate tissue (e.g., Primary Prostate Epithelial Cells, Normal, Human, ATCC PCS-440-010), supplement Prostate Epithelial Cell Basal Medium with the Prostate Epithelial Cell Growth Kit (ATCC PCS-440-040).
2. Optional media supplements:
   1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
   2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
   3. Phenol Red (ATCC PCS-999-001)

1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
2. Thaw the components of the growth kit just prior to adding them to the basal medium. If the growth kit contains L-glutamine, warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
3. Obtain one bottle of Prostate Cell Basal Medium (485 mL) from cold storage.
4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. Prostate Epithelial Cell Growth Kit Components   

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete media is summarized in Table 2.

Table 2. Addition of Antimicrobials/Antibiotics and Phenol Red (Optional)

6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
7. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days. 

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.