Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human

PCS-800-011 ^™

Primary Peripheral Blood Mononuclear Cells (PBMC) are a heterogeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes and lymphocytes. These cells were isolated from normal blood tissue and have been fully authenticated and characterized by ATCC. The cells can be used in the study of immunology, infection, cancer, hematology, and t-cell suppression assay. This one vial contains a minimum of 25 × 10⁶ viable cells (provided).

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Product category: Human cells
Product type: Primary cell
Organism: Homo sapiens, human
Cell type: mononuclear cell
Morphology: spherical; variable after culturing
Tissue: Blood
Disease: Normal
Applications: 3D cell culture

Immunology

Toxicology

Immuno-oncology

Cell and gene therapy (CGT) development
Product format: Frozen
Storage conditions: Vapor phase of liquid nitrogen

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Price: $265.00 EA

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Quantity	Lot Number	Inventory	Donor Age	Donor Sex	Donor Ethnicity	Donor BMI	CoA

Documentation

Product sheet

Application note

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

All tissues used for isolation are obtained under informed consent and conform to HIPAA regulations to protect the privacy of the donor’s Personally Identifiable Information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry Human immunodeficiency virus (HIV) and other bloodborne pathogens. With infectious virus assays or viral antigen assays, even a negative test result may not exclude the possibility of the existence of a latent viral genome or infectious viral particles below the lower limit of detection of that assay.

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Handle as a potentially biohazardous material using universal precautions. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Hank's Balanced Salt Solution (HBSS), 1X

30-2213

Fetal Bovine Serum (FBS)

30-2020

General

Specific applications: Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.

Characteristics

Cells per vial

≥ 2.5 x 10⁷

Volume

1.0 mL

Growth properties

Suspension, variable after culturing

Age

lot-specific

Ethnicity

Lot-specific

Gender

Lot-specific

Comments

Peripheral blood mononuclear cells (PBMC) have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.

Peripheral blood mononuclear cells are a heterogeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes and lymphocytes.

Handling information

Unpacking and storage instructions

Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium

Peripheral blood mononuclear cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.

Note: Application specific medium, cytokines and other factors

Handling procedure

Rapidly thaw cryovial in a 37°C water bath. Remove the vial before all the ice is thawed.
Decontaminate the cryovial with ethanol and move it to a biosafety cabinet.
While on ice, transfer contents of the vial to a 15 mL conical tube.
Rinse the cryovial with 1 mL of ice-cold thawing media (Hank's Balanced Salt Solution without Ca2+ or Mg2+ (ATCC 30-2213), supplemented with 10% Fetal Bovine Serum (ATCC 30-2020) and slowly transfer to the cells in the conical tube.
Slowly pipet 10X the vial volume of thawing media into the conical tube
Take a sample for counting and viability assessment.
Centrifuge the remaining contents of the vial at 4°C at 300 x g for 5 minutes to pellet cells.
Aspirate the wash leaving a few mL behind.
Re-suspend cells in the media of choice.

Subculturing procedure

N/A

Culture maintenance

N/A

Quality control specifications

Virus testing: Human T-lymphotropic virus 2 (HTLV-2): Not detected

Human immunodeficiency virus 1 (HIV-1): Not detected

West Nile virus (WNV): Not detected

Trypanasoma cruzi: Not detected

Hepatitis C virus (HCV): Not detected

Hepatitis B virus (HBV): Not detected

Human T-lymphotropic virus 1 (HTLV-1): Not detected

Human immunodeficiency virus 2 (HIV-2): Not detected
Viability: ≥ 70% when thawed from cryopreservation
Specific staining: Positive for CD45
Lot-specific expression of CD3, CD8, CD4, CD56, CD14, and CD19

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Aboul‐Enein M N, et al. Design, Synthesis, and Cytotoxic Evaluation of Certain 7‐Chloro‐4‐(piperazin‐1‐yl) quinoline Derivatives as VEGFR‐II Inhibitors. Arch Pharm Weinheim 350: 3-4, 2017. PubMed: 28304102

Hou W, et al. Determination of the Cell Permissiveness Spectrum, Mode of RNA Replication, and RNA-Protein Interaction of Zika Virus. BMC Infect Disease, 17(1):239, 2017. PubMed: 28359304

Mohamed, M, et al. Cytotoxicity And Anti-Cancer Effect Of Mangrove Crab (Scylla Serrata) Soup On Human Leukemic Jurkat Cells. Eur J Pharma Med Res 4(2): 192-195, 2017.

Li, X et al. Brain Natriuretic Peptide-Regulated Expression of Inflammatory Cytokines in Lipopolysaccharide (LPS)-Activated Macrophages Via NF-kB and Mitogen Activated Protein Kinase (MAPK) Pathways. Med Sci Monit, 24: 3119-3126, 2018. PubMed: 29754152

Kim Wiese, A, et al. DNA-PKcs Controls Calcineurin Mediated IL-2 Production in T Lymphocytes. PloS One, 12(7):e0181608, 2017. PubMed: 28750002

View All Curated Citations for this Product

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