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Waddlia chondrophila Everett et al.

VR-1470

Product category
Bacteria
Classification
Waddliaceae
Strain designation
WSU 86-1044
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation.
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate).
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp.
Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.

Characteristics

Comments
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. and 14 members of the genus Rickettsia all failed to react significantly. Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate).
Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.
MEM with 10% serum
Mycoplasma contamination
Not detected

Handling information

Host
Host Range: BT cells; P388D1 mouse macrophage cells
Effects
cell death; CPE; inclusions
Temperature
35°C
Atmosphere
95% Air, 5% CO2
Incubation
3 days at 35°C in BT cells (ATCC CRL-1390), 5% CO2
Handling notes
Reference antisera against Coxiella, Wolbachia, Chlamydia and Anaplasma spp. And 14 members of the genus Rickettsia all failed to react significantly.  Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate). Identification is recommended by 16S rRNA sequence--GenBank accession number AF042496.  Dense forms enter cytoplasm and reticulated forms develop.  These forms develop to dense forms that are released from the cell.
Key abbreviations
TCID[50], Median tissue culture infective dose
BT, Bovine turbinate cells

History

Deposited as
Waddlia chondrophila Everett et al.
Depositors
TF McElwain
Type of isolate
Animal
Special collection
NCRR Contract
Cross references
GenBank AF042496 16S ribosomal RNA and 23S ribosomal RNA, complete sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. Note: These are considered live cultures, not diagnostic or analytics samples. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Dilbeck PM, et al. Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J. Clin. Microbiol. 28: 814-816, 1990. PubMed: 2185269

Kocan KM, et al. Development of a rickettsia isolated from an aborted bovine fetus. J. Bacteriol. 172: 5949-5955, 1990. PubMed: 2211519

Rurangirwa FR, et al. Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov.. Int. J. Syst. Evol. Microbiol. 49: 577-581, 1999. PubMed: 10319478

type strain

Terry F McElwain, personal communication

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