Chlamydia suis Everett et al.
VR-1474 ™
- Product category
-
Bacteria
- Classification
- Chlamydiaceae
- Strain designation
- S45 (porcine isolate)
- Type strain
- Yes
- Isolation source
- Swine intestine, feces, and diarrhea fluid.
- Applications
-
Agricultural research
- Product format
- Frozen
- Storage conditions
-
Vapor phase of liquid nitrogen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Detailed product information
Characteristics
- Comments
- Please see ATCC Virology Guide for more detailed information on propagation
- Mycoplasma contamination
- Not detected
Handling information
- Host
-
HEp-2 (ATCC CCL-23)
- Effects
-
CPE; cytoplasmic inclusions
- Complete medium
-
Special Maintenance Medium (SMM): EMEM (ATCC® 30-2003™) + 10% prescreened FBS + 10 mM HEPES (Gibco 15630) + 2 µg/mL Cycloheximide (SIGMA C4859)
- Temperature
-
37°C
- Atmosphere
-
95% Air, 5% CO2
- Incubation
- Incubate infected culture for 72 hours at 37°C in a humidified 5% CO2 atmosphere.
- Recommendation for infection
- For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer.Sonicate seed material for 20 seconds at approximately 240W to disrupt cells. Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2). Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
- Handling notes
-
Cytoplasmic, iodine-positive inclusions in infected L or BGMk cells, elementary bodies in Gimenez-stained impression smears from infected yolk sacs of chicken embryos - biovar 5 and serovar 5 of animal chlamydiae - MOMP gene sequences of Chlamydia trachomatis - proposed for classification as Chlamydia suis sp. nov.
- Key abbreviations
-
EMEM, Eagle's Minimum Essential MediumPrescreened FBS, Fetal bovine serumscreened for Chlamydia inhibition due to antibody presenceg, Acceleration of gravityHEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)SMM, Special maintenance medium
History
- Deposited as
- Chlamydia suis Everett et al.
- Depositors
- J Storz
- Type of isolate
- Animal
- Special collection
-
NCRR Contract
- Cross references
-
GenBank AJ012176 Chlamydia suis partial rnpB gene for RNase P RNA subunit.GenBank AF269274 Chlamydia suis S45 major outer membrane protein (ompA) gene, partial cds; and tRNA-Gly gene, partial sequence.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors
For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. Note: These are considered live cultures, not diagnostic or analytics samples. We cannot ship this item until we receive this permit.
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462
Kaltenboeck B, et al. Structures of and allelic diversity and relationships among the major outer membrane protein (ompA) genes of the four chlamydial species. J. Bacteriol. 175: 487-502, 1993. PubMed: 8419295
type strain
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