Vaccinia virus

VR-1508 ^™

Vaccinia virus strain Modified vaccinia virus Ankara (MVA) is propagated in BHK-21 [C-13] cells (ATCC CCL-10). This strain was derived from the CVA (Ankara) strain via serial passage in chicken embryo fibroblasts followed by plaque purification 3 times.

This product requires a VS 16-6A permit, which can be downloaded at the USDA website here. Please submit the completed permit form to [email protected] with your ATCC sales order number and/or your purchase number noted in the subject line and body of the email.

Product category: Viruses
Product type: Animal virus
Classification: Poxviridae, Orthopoxvirus
Strain designation: Modified vaccinia virus Ankara (MVA)
Isolation source: CVA (Ankara) strain serially passaged in chicken embryo fibroblasts and plaque purified three times.
Applications: Infectious disease research
Product format: Frozen
Storage conditions: -70°C or colder

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Documentation

Product sheet

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

BHK-21 [C-13]

CCL-10

Eagle's Minimum Essential Medium (EMEM)

30-2003

Fetal Bovine Serum (FBS)

30-2020

General

Specific applications: Pox virus research

Characteristics

Comments: Does not replicate in human cells or the majority of mammalian cells. This is a highly attenuated strain that some experts feel can be handled safely by trained personnel without the need for vaccination.
Mycoplasma contamination: Not detected

Handling information

Host: BHK-21 [C-13] (ATCC CCL-10)
Effects: cell rounding; cell sloughing; CPE
Complete medium: EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature: 37°C
Atmosphere: 95% Air, 5% CO₂
Incubation: 3-7 days
Recommendation for infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes: Does not replicate in human cells or the majority of mammalian cells. This is a highly attenuated strain that some experts feel can be handled safely by trained personnel without the need for vaccination.
Key abbreviations: FBS, Fetal bovine serum

MOI, Multiplicity of infection

°C, Degrees Celsius

CO₂, Carbon dioxide

CPE, Cytopathic effect

CVA, Chorioallantoic vaccinia Ankara

EMEM, Eagle's Minimum Essential Medium

Quality control specifications

Volume: 1.0 mL

History

Deposited as: Vaccinia virus
Depositors: B Moss, LS Wyatt, National Institute of Allergy and Infectious Diseases
Chain of custody: ATCC <-- B Moss, LS Wyatt, National Institute of Allergy and Infectious Diseases <-- A Mayr
Type of isolate: Animal
Special collection: NCRR Contract
Cross references: GenBank U94848 Vaccinia virus strain Ankara, complete genomic sequence

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. Note: These are considered live cultures, not diagnostic or analytics samples. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Antoine G, et al. The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses. Virology 244: 365-396, 1998. PubMed: 9601507

Bernard Moss, personal communication

Mahnel H, Mayr A. Experiences with immunization against orthopox viruses of humans and animals using vaccine strain MVA. Berlin Munch. Tierarztl. Wochenschr. 107: 253-256, 1994.

Meyer H, et al. Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence. J. Gen. Virol. 72: 1031-1038, 1991. PubMed: 2033387

Sutter G, Moss B. Nonreplicating vaccinia vector efficiently expresses recombinant genes. Proc. Natl. Acad. Sci. USA 89: 10847-10851, 1992. PubMed: 1438287

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