Chlamydia pneumoniae Grayston et al.

VR-2282 ^™

Chlamydia pneumoniae strain TW-183 is propagated in HEp-2 cells (ATCC CCL-23). This strain was isolated in 1965 from the conjunctiva of a child in Taiwan. It has applications in respiratory and infectious disease research.

Product category: Bacteria
Classification: Chlamydiaceae
Strain designation: TW-183
Type strain: Yes
Former name: Chlamydophila pneumoniae (Grayston et al.) Everett et al.
Isolation source: Conjunctiva of a child
Geographical isolation: Taiwan
Applications: Infectious disease research

Respiratory disease research
Product format: Frozen
Storage conditions: -70°C or colder

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Documentation

Product sheet

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

HEp-2

CCL-23

Dulbecco's Modified Eagle's Medium (DMEM)

30-2002

General

Specific applications: Respiratory research

Characteristics

Comments: Note that activities with high potential for aerosol production require BSL-3 facilities and practices.

For a time, prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were referred to as TWAR, after the isolates TW-183 and AR-39. AR-39 is available as ATCC 53592.

The inclusions are iodine stain negative (contain no glycogen).

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.

Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 37°C for 72 hours.

Infection of TC is enhanced by subconfluent monolayer of cells.

This preparation has tested positive for Mycoplasma contamination.

The inclusions are iodine stain negative (contain no glycogen). The TWAR strains (now C. pneumoniae) of Chlamydia are named after TW183 and AR39.
Mycoplasma contamination: Detected
Technical information: ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Host: HEp-2 (ATCC CCL-23)
Effects: CPE; cytoplasmic inclusions
Complete medium: DMEM (ATCC 30-2002) + 10% prescreened FBS + 10 mM HEPES + 2 µg/mL Cycloheximide (Sigma C-4859 Ready-Made)
Temperature: 35°C
Atmosphere: 95% Air, 5% CO₂
Incubation: 3 days at 35°C, a 5% CO₂ in air atmosphere is recommended
Recommendation for infection: For best results cells should be 24 to 48 hours old and 90% - 100% confluent
Handling notes: Note that activities with high potential for aerosol production require BSL 3 facilities and practices. This preparation has tested positive for Mycoplasma contamination. For a time, prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were referred to as TWAR, after the isolates TW-183 and AR-39. AR-39 is available as ATCC^® 53592™. The inclusions are iodine stain negative (contain no glycogen). Suggested protocol for propagation: Add glassbeads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 35°C for 3 days. Infection of TC is enhanced by subconfluent monolayer of cells. This preparation has been tested and found to be positive for Mycoplasma contamination.
Key abbreviations: °C, Degrees Celsius

AR-39, TWAR Chlamydia strain

CO₂, Carbon dioxide

DMEM, Dulbecco's Modified Eagle's Medium

FBS, Fetal bovine serum

g, Acceleration of gravity

HEp-2, Human laryngeal tumor cells

HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)

μg, Microgram

uM, micromolar

mg, Milligram

mL, Milliliter

mM, millimolar

rpm, Revolutions per minute

TW-183, TWAR Chlamydia strain

TWAR, Taiwan Acute Respiratory

History

Deposited as: Chlamydia pneumoniae Grayston et al.
Depositors: G Galloway, T Grayston
Patent depository: This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number: 5,008,186
Type of isolate: Human
Year of origin: 1965
Special collection: NCRR Contract
Cross references: GenBank AJ012174 Chlamydophila pneumoniae partial rnpB gene for RNase P RNA subunit.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Sachse K, et al. Emendation of the family Chlamydiaceae: proposal of a single genus, Chlamydia, to include all currently recognized species. Syst Appl Microbiol 38(2): 99-103, 2015. PubMed: 25618261

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