Quantitative genomic RNA from Severe acute respiratory syndrome-related coronavirus 2 strain USA/CA_CDC_5574/2020 (variant B.1.1.7)

Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/CA_CDC_5574/2020.

This isolate is lineage B.1.1.7, clade GR according to GISAID (Global Initiative on Sharing All Influenza Data) conventions.

The following mutations were identified in the clinical isolate: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del, M (Membrane protein) V70L, N (Nucleocapsid protein) D3L, N G204R, N R203K, N S235F, NS3 T223I, NS8 (Non-structural protein 8) Q27stop, NS8 R52I, NS8 Y73C, NSP3 (Non-structural protein 3) A890D, NSP3 I1412T, NSP3 T183I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) A454V, NSP13 K460R.

One additional SNP in ORF1ab L3826F was reported in the deposited passage two virus, in comparison to the clinical specimen.

Genomic RNA from SARS-CoV-2 must be handled at BSL-2 containment.

Innocuity testing was performed on the extracted RNA and results reported on the Certificate of Analysis.

Acknowledgment for publications should read "The following reagent was deposited by the Centers for Disease Control and Prevention and obtained through BEI Resources, NIAID, NIH: Genomic RNA from SARS-Related Coronavirus 2, Isolate USA/CA_CDC_5574/2020 (Lineage B.1.1.7), NR-55244."

1. Thaw the vial on ice. Avoid exposing the RNA to repeated freeze-thaw cycles as it may result in degradation.
2. Gently mix the sample to ensure an even distribution of material.
3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.

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