Mycobacterium nonchromogenicum Tsukamura

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #1395 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1395 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #173 plate and/or #173 or #90 agar slant.
4. Incubate at 37°C for 8 to 10 days.

Colonies on #173 agar are small, slightly irregular, entire, low convex, translucent, cream-colored, and dull. Growth on solid medium takes up to 10 days.

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