Parvimonas micra (Prévot) Tindall and Euzéby

1. Open thawed vial.
2. Under anaerobic conditions aseptically transfer the entire contents to a 5-6 mL tube of #1015 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
3. Use several drops of the primary broth tube to inoculate a #260 plate.
4. Incubate in an anaerobic atmosphere at 37°C for 72 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

• Loose screw caps on test tubes in an anaerobic chamber
• Loose screw caps on test tubes in an activated anaerobic gas pack jar
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

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