Lacticaseibacillus paracasei (Collins et al.) Zheng et al.

1. Open vial.
2. Using a single tube of #416 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #416 slant and/or plate.
5. Incubate the tubes and plate at 37°C aerobically or in an atmosphere of 5% CO₂ for 48 hours.

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