Nonomuraea roseola (Lavrova and Preobrazhenskaya) Zhang et al.

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #551 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #551 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #551 plate and/or #551 agar slant.
4. Incubate at 28°C for 14 days.

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