Leptospira weilii Yasuda et al.

1. Transfer immediately upon thawing by aseptically withdrawing cell suspension  from vial and inoculating a 10-12 mL tube of Medium #1470, inserting the pipette just below the surface of the semi-solid medium.  Aliquots of 0.5 mL from this tube may be used to inoculate additional tubes if needed. An aerobic blood plate may also be streaked to test for purity. 
2. Incubate test tubes with screw caps lightly tightened along  with the blood plate at 30°C under aerobic conditions. 
3. Initial growth may take 1- 4 weeks. Observe growth as a thin, tight band forming just below the surface of the medium. The band thickens as incubation continues. Cellular morphology under phase contrast shows active, spiral cells with flexing motility. 
4. The aerobic blood plate should show no signs of growth.

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