Thiothrix fructosivorans Howarth et al.
49748 ™
49748 ™
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2. Open the frozen vial and aseptically transfer the contents into the 125 ml flask.
3. Incubate flask at 10°C for 4-6 days and double the volume with the addition of 10 ml of 1820 medium.
4. Once growth has been established transfer flask contents to 100 ml of #1820 broth contained in a 250 ml Erlenmeyer flask. Incubate flask without shaking at 10°C. Growth will be evident by the presence of a stringy flocculent which can take as little as 14 days or as long as 21 days to appear.
5. Transfer to fresh medium at 2 month intervals. A 5% inoculum (5ml of culture to 100 ml fresh medium) is recommended.
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Williams TM, Unz RF. Filamentous sulfur bacteria of activated sludge: characterization of Thiothrix, Beggiatoa, and Eikelboom type 021N strains. Appl. Environ. Microbiol. 49: 887-898, 1985. PubMed: 4004221
. . Water Res. 23: 15-22, 1989.
Williams TM, et al. Ultrastructure of Thiothrix spp. and "type 021N" bacteria. Appl. Environ. Microbiol. 53: 1560-1570, 1987.
Howarth R, et al. Phylogenetic relationships of filamentous sulfur bacteria (Thiothrix spp. and Eikelboom type 021N bacteria) isolated from wastewater-treatment plants and description of Thiothrix eikelboomii sp. nov., Thiothrix unzii sp. nov., Thiothrix fructosivorans sp. nov. and Thiothrix defluvii sp. nov.. Int. J. Syst. Bacteriol. 49: 1817-1827, 1999. PubMed: 10555365
type strain