Methylophilus methylotrophus Jenkins et al.

Potential strain for single cell protein production for food or feed

1. Open vial according to enclosed instructions.
2. Using a single tube of #1545 broth (5 to 6 mL), aseptically transfer 0.5 to 1.0 mL to the vial and rehydrate the pellet.           
3. Aseptically transfer the rehydrated pellet back into the broth tube and mix well.  Transfer 0.1 mL to slants.  Plate 0.1 mL of the culture onto a non-selective medium (plate) to test for purity.   
4. Incubate the culture at 30°C for 4 to 5 days.  Incubate non-selective plate aerobically.     
5. Growth should be detected within 3 to 5 days.  Once growth has been detected transfer the culture to fresh broth tubes and slants.  No growth should occur on non-selective plate.                       

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