Desulfovibrio aespoeensis Motamedi and Pedersen
700646 ™
700646 ™
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2. Perform all steps under anaerobic conditions. (see below)
3. Aseptically transfer 0.5 ml of ATCC Medium #2108 to the vial and rehydrate the freeze-dried pellet. Transfer the suspension back into the tube of broth. Inoculate a plate of non-selective medium with 0.1 of the culture.
4. Seal the test tube with a rubber stopper and incubate anaerobically at 30oC. Incubate the plate(s) aerobically as a purity check.
5. Growth could be detected within 24 hours. Cells appear as vibriod shaped rods in singles and pairs. The cells are motile. Good growth is not obtained on agar.. Once growth has been established, the culture should be transferred to fresh broth every 24 to 48 hours.
ANAEROBIC CONDITIONS:
· Tubes of media are placed under a gassing cannula system hooked to a source of oxygen free gas.
· All transfers are performed while the test tubes are on the cannula system with a gentle stream of oxygen-free gas flowing through the system.
· As the test tubes are removed from the cannula system each is sealed with butyl rubber stopper thus maintaining the anaerobic headspace.
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Motamedi M, Pedersen K. Desulfovibrio aespoeensis sp. nov., a mesophilic sulfate-reducing bacterium from deep groundwater at Aspo hard rock laboratory, Sweden. Int. J. Syst. Bacteriol. 48: 311-315, 1998. PubMed: 9542102
type strain