EB-3 [EB3] (ATCC® CCL-85)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
[Cells Contain HERPESVIRUS]
Disease Burkitt's lymphoma
Age 3 years
Gender Male
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Black
3 years
Antigen Expression
HLA A3, Aw32, Cw2
Virus Resistance
partially resistant to polioviruses and vesicular stomatitis virus
Comments
EBNA positive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 x 105 viable cells/mL.
Cryopreservation
culture medium, 90%; DMSO, 10%
Culture Conditions

Temperature: 37.0°C

Atmosphere: air, 95%. carbon dioxide (CO2), 5%

STR Profile
Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
Isoenzymes
G6PD, A
Name of Depositor W Henle
References

Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852

. . Trans. N.Y. Acad. Sci. 29: 71, 1966.

. . Wistar Inst. Symp. Monogr. 4: 59, 1965.

Basic Documentation
References

Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852

. . Trans. N.Y. Acad. Sci. 29: 71, 1966.

. . Wistar Inst. Symp. Monogr. 4: 59, 1965.