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ABRF-MGRG 10 Strain Even Mix Genomic Material (ATCC® MSA-3001)

Permits and Restrictions

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10.0% Bacillus subitilis (ATCC 23857)
10.0% Chromobacter violaceum (ATCC 12472)
10.0% Entercoccus faecalis (ATCC 47077)
10.0% Escherichia coli (ATCC 700926)
10.0% Halobacillus halophilus (ATCC 35676)
10.0% Haloferax volcanii (ATCC 29605)
10.0% Micrococcus luteus (ATCC 4698)
10.0% Pseudoalteromonas haloplanktis (ATCC 14393)
10.0% Pseudomonas fluorescens (ATCC 13525)
10.0% Staphylococcus epidermidis (ATCC 12228)
Applications ATCC® Microbiome Standards enable the optimization of metagenomics workflows and microbiome research applications, providing reliable comparative data while improving assay consistency.

This product is ideal for environmental analyses, assay development, and as a daily run control for molecular diagnostics and microbiome applications.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments ATCC® Microbiome Standards are mock microbial communities that mimic mixed metagenomic samples. The ABRF-MGRG metagenomics reference standard was developed and packaged in partnership with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics Research Group (MGRG)*, an international scientific society dedicated to advancing technologies, education, communication, and reproducible research in the operations of shared scientific resources, as part of the Extreme Microbiome Project (XMP). This product comprises genomic DNA from fully sequenced**, characterized, and authenticated bacterial and archaeal species observed in various environments, including soil, freshwater, seawater, feces, and high salinity ecosystems. Further, this reference community was derived from Class I microbial genomes (Koren et al., Genome Biol 14(9):R101, 2013), was extracted to retain long fragments of gDNA, and is confirmed to be free of human DNA. Each order for this product is provided with access to run 20 analyses on One Codex, the leading bioinformatics platform for microbial genomics and metagenomics.

Specification Range: 5.26 ng/µL to 8.76 ng/µL***

*The bulk material for this product was deposited by ABRF-MGRG. The research group extracted DNA from each individual strain and mixed them in appropriate proportions.
**The reference genome for Pseudoalteromonas haloplanktis is not available
**DNA concentrations indicate ATCC manufacturing specifications and are provided as a reference only.
Product Format frozen 50 µL in Tris-HCl pH 8.5
Storage Conditions -20°C or colder
Intended Use

Purchase of this product will include limited use access to the One Codex platform for metagenomic analysis of ATCC microbiome products. By purchasing this product, you consent and agree to allow ATCC to securely transfer sales and end users’ data related to this product to One Codex. Provision of One Codex access, storage, management of sequencing data, and the interpretation and accuracy of analytical results provided by One Codex are the responsibility of One Codex and not ATCC. End users should visit for their complementary metagenomic data analysis.

One Codex
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
  1. ATCC Microbiome Standard - How to Perform an Analysis with One Codex

    Date Updated: 7/12/2019