Human respiratory syncytial virus (ATCC® VR-1540)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 1 mL per vial

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Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus
Strain A2
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images Human respiratory syncytial virus ATCC VR-1540 images
Comments VR-1540 was prepared from ATCC VR-1302; by growth in the presence of neutralizing serum to remove contaminating Human adenovirus 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Recommended Host
HEp-2 (ATCC CCL-23)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Name of Depositor L Potash, Flow Laboratories, Inc.; ATCC
Special Collection NCRR Contract
Source Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961

Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423

Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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