Mycobacterium aromaticivorans

1. Open vial according to enclosed instructions. 
2. Using a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #173 agar slant and/or plate.
5. Incubate the tubes and plate at 30^oC for one week.

Colonies on #173 agar are circular, entire, creamy, and becomes light yellow as culture ages.  This strain will also grow on TSA agar; however growth will not be as abundant.

Additional information on this culture is available on the ATCC web site at www.atcc.org.

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