HUV-EC-C [HUVEC]
CRL-1730 ™
CRL-1730 ™
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The base medium for this cell line is F-12K Medium (ATCC 30-2004). To make the complete growth medium, add the following components to 450 mL of the base medium:
Note: Because of limited stability, the ECGS should be added to an aliquot of the above culture medium fresh prior to seeding or performing fluid changes. Complete media supplemented with the below item expires 7 days after preparation.
To prepare 30 mg/mL ECGS stock solution, aseptically combine:
Store in working aliquots at -20°C. ECGS is stable for 1 month when prepared and stored as directed. Do not repeat freeze/thaw.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning T-75 flasks (catalog #430641U) are recommended for subculturing this product.
Note: A high quality ECGS prepared from bovine neural tissue (BD Biosciences catalog # 354006 or equivalent) should be used to propagate CRL-1730. It is best to initiate the cells with the highest recommended concentration of ECGS. Moderate to heavy debris and numerous floating cells may be routinely observed in cultures of HUV-EC-C cells. Retain the floating cells by gentle centrifugation and add back to the adherent population.
Cultures should be fully fluid changed every 48 hours. The cells should only be allowed to go 72 hours without fluid changing when the density is less than 50% confluent. Perform full fluid changes rather than media additions.
This cell line produces a lot of floaters and debris especially at higher densities. Cells detach before completely filling in to 100% confluence. It is recommended to subculture the cells when 80 to 90% confluent to avoid excessive floaters. Floating cells are viable and if pronounced, they should be spun down and reseeded back into the growing culture.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week
Seeding Density: 8.0 x 103 to 3.0 x 104 viable cells/cm2
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
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