SB-1014 ™
The vector contains a LacZ alpha gene flanked by two BsaI sites that is inserted between two att sites (att L4 and att R1) for a Golden Gate-compatible cloning step to insert any promoter of interest (Detailed information is described in the ATCC® Synthetic Biology Solutions User Guide).
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
An empty entry vector of the Gateway® system for adding any promoter into the promoter library in the ATCC® Synthetic Biology Yeast Tool Kit. Gateway® is a registered trademark of Thermo Fisher Scientific.
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
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