TSD-298 ™
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Depositor recommends growth on Middlebrook 7H10 medium supplemented with 0.5% glycerol and 10% OADC-oleic acid, albumin, dextrose and catalase (Becton Dickinson).
Strain was cultivated in solid medium to observe macroscopic characteristics of individual colonies which were compatible with a single profile. Analysis of the gene sequences (rpoB, hsp65 and 16S) showed no contamination. To verify possible genomic contamination, we used Kraken2 v2.0.8_beta with the database ftp://ftp.ccb.jhu.edu/pub/data/kraken2_dbs/minikraken_8GB_202003.tgz.
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