Heat-inactivated SARS-CoV-2, Omicron variant

This isolate is lineage BA.5.5 (Pango v.4.1.3 PLEARN-v1.17), Omicron (BA.5-like) (Scorpio), and GISIAD clade GRA using the Phylogenetic Assignment of Named Global Outbreak lineages (PANGO) tool.  Due to the dynamic nature of lineage determination using PANGO, discrepancies may exist between vial labels and the current lineage assignment.  Previous iterations of PANGO identified this isolate as lineage BA.5.

The complete genome of the clinical isolate of SARS-CoV-2, USA/COR-22-063113/2022 has been sequenced (GISAID: EPI_ISL_13512579). The following mutations are present in the clinical isolate: Spike A27S, Spike D405N, Spike D614G, Spike D796Y, Spike E484A, Spike F486V, Spike G142D, Spike G339D, Spike H69del, Spike H655Y, Spike K417N, Spike L24del, Spike L452R, Spike N440K, Spike N501Y, Spike N679K, Spike N764K, Spike N969K, Spike P25del, Spike P26del, Spike P681H, Spike Q498R, Spike Q954H, Spike R408S, Spike S371F, Spike S373P, Spike S375F, Spike S477N, Spike T19I, Spike T76I, Spike T376A, Spike T478K, Spike V70del, Spike V213G, Spike Y505H, E T9I, M A63T, M D3N, M Q19E, N E31del, N G204R, N P13L, N R32del, N R203K, N S33del, N S413R, NS3 T223I, NSP1 S135R, NSP3 G489S, NSP3 T24I, NSP4 L264F, NSP4 T327I, NSP4 T492I, NSP5 P132H, NSP5 P252L, NSP6 F108del, NSP6 G107del, NSP6 S106del, NSP12 P323L, NSP13 R392C, NSP14 I42V, NSP15 T112I. It was labelled as variant of concern (VOC) Omicron by the World Health Organization (WHO).

This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/COR-22-063113/2022 that has been inactivated by heating at 65°C for 30 minutes and is therefore unable to replicate.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.

This isolate is lineage BA.5.5 (Pango v.4.1.3 PLEARN-v1.17), Omicron (BA.5-like) (Scorpio), and GISIAD clade GRA using the Phylogenetic Assignment of Named Global Outbreak lineages (PANGO) tool.  Due to the dynamic nature of lineage determination using PANGO, discrepancies may exist between vial labels and the current lineage assignment.  Previous iterations of PANGO identified this isolate as lineage BA.5.

The complete genome of the clinical isolate of SARS-CoV-2, USA/COR-22-063113/2022 has been sequenced (GISAID: EPI_ISL_13512579). The following mutations are present in the clinical isolate: Spike A27S, Spike D405N, Spike D614G, Spike D796Y, Spike E484A, Spike F486V, Spike G142D, Spike G339D, Spike H69del, Spike H655Y, Spike K417N, Spike L24del, Spike L452R, Spike N440K, Spike N501Y, Spike N679K, Spike N764K, Spike N969K, Spike P25del, Spike P26del, Spike P681H, Spike Q498R, Spike Q954H, Spike R408S, Spike S371F, Spike S373P, Spike S375F, Spike S477N, Spike T19I, Spike T76I, Spike T376A, Spike T478K, Spike V70del, Spike V213G, Spike Y505H, E T9I, M A63T, M D3N, M Q19E, N E31del, N G204R, N P13L, N R32del, N R203K, N S33del, N S413R, NS3 T223I, NSP1 S135R, NSP3 G489S, NSP3 T24I, NSP4 L264F, NSP4 T327I, NSP4 T492I, NSP5 P132H, NSP5 P252L, NSP6 F108del, NSP6 G107del, NSP6 S106del, NSP12 P323L, NSP13 R392C, NSP14 I42V, NSP15 T112I. It was labelled as variant of concern (VOC) Omicron by the World Health Organization (WHO).

This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/COR-22-063113/2022 that has been inactivated by heating at 65°C for 30 minutes and is therefore unable to replicate.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

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