Abstract
Storage of microorganisms in a quantitative format is essential for modern industrial quality control and other related applications. Lyophilization, or freeze drying, is one of the most widely used techniques for preserving microorganisms. However, lyophilization is a complex process that involves phase changes of the extracellular environment, and the outcome is highly influenced by the compositional, thermal, and pressure changes of the materials. During lyophilization, cellular stress creates changes in the global protein profile and might also cause genetic variability. We have used global proteomic analysis (LC-MS/MS) and next-generation sequencing to evaluate the impact of lyophilization on the viability of microorganisms. In this study, we used Escherichia coli (ATCC 8739) as a model organism to evaluate how changes in the lyophilization formulation affect protein expression characteristics. Our findings underscore the need to use a proper lyophilization formulation and profile for optimum viability and less genetic variability.
Download the presentation to learn how lyophilization formulations can affect bacterial viability and genetic variability.
DownloadPresenter
Jyoti K Jha, PhD
Senior Scientist, BioNexus Cryobiology, ATCC
Dr. Jyoti K. Jha is a research and development scientist with over 15 years of experience in molecular biology and biochemistry of microbes. He previously worked at Elanco and Rise Therapeutics as a senior scientist for microbial product development. Currently, Dr. Jha develops and improves the mode of microbial preservation formulation and format for new products. Dr. Jha received a PhD from the University of Calcutta in Biotechnology and performed post-doctoral work at National Institutes of Health (NIH/NCI). Dr. Jha has published more than 25 peer reviewed papers as lead and/or co-author.
